五月丁香六月婷婷综合,丰满少妇被激烈进入在线播放,ssav在线观看,在这无码av另类网

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>大鼠正常細(xì)胞>CRL-1444A7r5 大鼠胸大動(dòng)脈平滑肌細(xì)胞

A7r5 大鼠胸大動(dòng)脈平滑肌細(xì)胞

簡(jiǎn)要描述:CRL-1444 A7r5 大鼠胸大動(dòng)脈平滑肌細(xì)胞, ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件

  • 產(chǎn)品型號(hào):CRL-1444
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-14
  • 訪  問(wèn)  量:2489

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-1444 A7r5 大鼠胸大動(dòng)脈平滑肌細(xì)胞, ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)優(yōu)培養(yǎng)條件

CRL-1444 A7r5 大鼠胸大動(dòng)脈平滑肌細(xì)胞的詳細(xì)介紹


ATCC® Number:CRL-1444™  Price:$275.00
Designations:A7r5

Depositors:W Carlisle

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Rattus norvegicus (rat)

Morphology:fibroblast

Source:Organ: aorta, thoracic
Strain: DB1X
Tissue: smooth muscle


Cellular Products:myokinase; creatine phosphokinase (muscle isoenzyme); myosin

Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
CRL-1444

Applications:transfection host (Roche FuGENE® Transfection Reagents

technology from amaxa)



Age:embryo

Comments:The cells exhibit an increase in activity of the enzymes myokinase and creatine phosphokinase (CPK) as the culture reaches stationary phase.

Muscle type CPK is synthesized after cell division has ceased.



Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C


Subculturing:Protocol:
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:3 is recommended
Medium Renewal: Every 2 to 3 days


Preservation:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase


Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020



References:1061: Kimes BW, Brandt BL. Characterization of two putative smooth muscle cell lines from rat thoracic aorta. Exp. Cell Res. 98: 349-366, 1976. PubMed: 943301

CRL-1444




















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫(xiě)阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
久久蜜臀| 妓女一区二区三区在线观看| 亚洲综合色久av| 先锋国产资源| 深夜a级毛片免费视频| 黄本网站久久久久| 国产丝袜二区在线播放| 久久AV电影频道| 亚洲老鸭窝一区二区| 国产精品中文字幕电影| 日韩欧美-区二区| 亚洲精品色丁香五月| 国产成人愉拍精品| 网暗稀缺拗女姿源在线| 国产久艹视频在线观看| 久久久黄色录像| 91蝌蚪91九色| 日韩精品一区二区免费视频在线| 张开腿狠狠操| 青草久久香| 久久精品久久精品国产大片| 亚洲欧美日韩专区| 国产成人久久综合一区| 日本久久aⅴ成人精| 浪潮AV色综合久久| 国产精品午夜福利麻豆| 亚洲aⅴ无码片一区二区三区| 亚洲九九综合网| 久久精品女人毛片国产| 日本在线播放xxx| 国产又粗又猛又爽的网站| 精品国产爽香蕉在线观看| 国产精品xxxxXc| 91色老久久精品偷偷蜜臀| 国产欧美日韩精品久久久久久| 精品人妻无码久| 亚洲无码AV一区二区| 人妻中文日韩无码| 亚洲中文字幕在线看| 精品婷婷一区二区三区| 97国产视频在|