五月丁香六月婷婷综合,丰满少妇被激烈进入在线播放,ssav在线观看,在这无码av另类网

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人腫瘤細(xì)胞、癌細(xì)胞>CRL-2557Panc 05.04細(xì)胞, 人胰腺癌細(xì)胞

Panc 05.04細(xì)胞, 人胰腺癌細(xì)胞

簡要描述:Panc 05.04細(xì)胞, 人胰腺癌細(xì)胞
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

  • 產(chǎn)品型號:CRL-2557
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2024-11-21
  • 訪  問  量:1552

產(chǎn)品分類

Product Category

詳細(xì)介紹

Panc 05.04細(xì)胞, 人胰腺癌細(xì)胞

ATCC® Number:CRL-2557™    Price:$417.00
Designations:Panc 05.04Depositors:EM JaffeeBiosafety Level:1Shipped:frozenMedium & Serum:See PropagationGrowth Properties:adherentOrganism:Homo sapiens (human)Morpholoepithelial Source:gy:Organ: pancreas Disease: adenocarcinomaCellular Products:cytokeratins 7 and 18 [50655]Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.Panc 05.04細(xì)胞, 人胰腺癌細(xì)胞Isolation:Isolation date: May 5, 1995Tumorigenic:YesOncogene:K-ras +Antigen Expression:MHC class I +; MHC class II - [50655] Blood type B; Rh+Age:77 years adultGender:femaleEthnicity:WhiteComments:Panc 05.04 is a pancreatic adenocarcinoma epithelial cell line derived, in 1995, from a primary tumor removed from the head-of-the-pancreas of a female with pancreatic adenocarcinoma. The cell line exhibits a K-ras oncogene mutation at codon 12 where a GGT --> GAT mutation resulted in substitution of aspartic acid for glycine. [50655] The cells have a reported plating efficiency of 100%. [50655]Propagation:ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 20 Units/ml human recombinant insulin, 85%; fetal bovine serum, 15%Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°CSubculturing:Protocol:                    Remove and discard culture medium.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.To remove trypsin-EDTA solution, transfer cell suspension to centrifuge tube and spin at approximay 125 xg for 5 to 10 minutes.Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels.Incubate cultures at 37°C.Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:5 is recommended Medium Renewal: Add media once per week. Fluid change one to two times per week.Preservation:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phaseDoubling Time:46 hrsRelated Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001recommended serum:ATCC 30-2020References:50655: Jaffee EM, et al. Development and characterization of a cytokine-secreting pancreatic adenocarcinoma vaccine from primary tumors for use in clinical trials. Cancer J. Sci. Am. 4: 194-203, 1998. PubMed: 9612602

















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補充說明:

  • 驗證碼:

    請輸入計算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
一色桃子人妻成熟系列av| 韩国毛片| 日本中文字幕视频在线| 国产真实乱免费视频| 国产午夜成人精品福利在线观看| AV只有精品| 中文字幕一区二区三区在线乱码| www.自慰网| 澳门美女一级毛片大全| 日本一区二区黄色| 中文字幕免费观看一区三区| 99二区一区| 国模蓉蓉人体337p| 日韩不卡一区二区三区| 日韩精品一区二区三区免费高清| 日韩成人免费视频观看| 成人无码在线观看亚洲| 久久精品日韩一区| www.人人操| 综合久久精品色| 99国产普通自拍| 噜噜欧美| 国产精品视频偷伦精品视频| 东京热欧洲无码| 中国熟女黄色视频| 久久精品久久精品中文字幕| 欧美激情亚洲一区中文字幕| 伊人国内精品在线| 私库久久精品视频| 九re国产视频| 又黄又爽又湿又免费的视频| 人妻精品漫画| 香蕉国产线看观看| 亚洲一区二区高新区| 欧洲一区二区AV| 亚洲成a人片在线观看的电影手机版| 四虎网址在线看| 国产成人精品午夜在线播放| 正在播放后入内射少妇| 欧美日韩一区二区三区在线亚洲| 日本在线看片免费人成视频100|