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293 人胚腎細胞

簡要描述：CRL-1573 293 人胚腎細胞,ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞|貼壁細胞|懸浮細胞|。細胞庫管理規(guī)范，提供的細胞株背景清楚，提供參考文獻和培養(yǎng)條件

  • 產(chǎn)品型號：CRL-1573
  • 廠商性質：生產(chǎn)廠家
  • 更新時間：2024-11-16
  • 訪  問  量：2059

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詳細介紹

CRL-1573 293 人胚腎細胞


Designations:293

Depositors:FL Graham

Biosafety Level:2 [Cells contain adenovirus ]

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Homo sapiens

Morphology:CRL-1573 293 人胚腎細胞


Source:Organ: embryonic kidney

Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Restrictions:These cells are distributed for research purposes only. 293 cells, their products, or their derivatives may not be distributed to third parties.

Applications:efficacy testing

transfection host

viruscide testing



Receptors:vitronectin, expressed

Tumorigenic:YES

DNA Profile (STR):Amelogenin: X

CSF1PO: 11,12

D13S317: 12,14

D16S539: 9,13

D5S818: 8,9

D7S820: 11,12

THO1: 7,9.3

TPOX: 11

vWA: 16,19



Cytogenetic Analysis:This is a hypotriploid human cell line. The modal chromosome number was 64, occurring in 30% of cells. The rate of cells with higher ploidies was 4.2 %. The der(1)t(1;15) (q42;q13), der(19)t(3;19) (q12;q13), der(12)t(8;12) (q22;p13), and four other marker chromosomes were common to most cells. Five other markers occurred in some cells only. The marker der(1) and M8 (or Xq+) were often paired. There were four copies of N17 and N22. Noticeably in addition to three copies of X chromosomes, there were paired Xq+, and a single Xp+ in most cells.

Age:fetus

Comments:Although an earlier report suggested that the cells contained Adenovirus 5 DNA from both the right and left ends of the viral genome [RF32764], it is now clear that only left end sequences are present.

The line is excellent for titrating human adenoviruses.

The cells express an unusual cell surface receptor for vitronectin composed of the integrin beta-1 subunit and the vitronectin receptor alpha-v subunit.

The Ad5 insert was cloned and sequenced, and it was determined that a colinear seqment from nts 1 to 4344 is integrated into chromosome 19 (19q13.2).



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