五月丁香六月婷婷综合,丰满少妇被激烈进入在线播放,ssav在线观看,在这无码av另类网

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>動(dòng)物巨噬細(xì)胞>CRL-28433D4/21 豬肺泡巨噬細(xì)胞

3D4/21 豬肺泡巨噬細(xì)胞

簡要描述:CRL-2843 3D4/21 豬肺泡巨噬細(xì)胞
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件

  • 產(chǎn)品型號:CRL-2843
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-14
  • 訪  問  量:4202

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-2843   3D4/21  豬肺泡巨噬細(xì)胞

ATCC 細(xì)胞|細(xì)胞株|細(xì)胞系|腫瘤細(xì)胞|巨噬細(xì)胞

3D4/21 (ATCC® CRL-2843™)

Organism Sus scrofa, pig

Tissue  lung

Cell Type  macrophage macrophage (alveolar); immortalized with SV40 large T antigen transformed with pSV3-neo

Product Format  frozen

Morphology  macrophage

Culture Properties  adherent

Biosafety Level  2  [Cells contain SV40 viral DNA sequences]

Age  27 days

Gender  unknown

CRL-2843   3D4/21  豬肺泡巨噬細(xì)胞

Strain  Landrace

Applications

These porcine myelomonocytic cell lines may have a wide variety of applications in porcine virology and immunology Ref.



Storage Conditions  liquid nitrogen vapor phase

Derivation  The parental porcine monomyeloid cell line, 3D4, was established in December of 1998 following transfection of primary porcine alveolar macrophage cultures with the pSV3neo plasmid.


Single cell cloning and selection in G-418 of the 3D4 parental cell line resulted in establishment of 3D4/2 (ATCC CRL-2845), 3D4/21 (ATCC CRL-2843) and 3D4/31 (ATCC CRL-2844).

Virus Susceptibility  Bovine adenovirus 3

Classical swine fever virus , Classical swine fever virus

Human parainfluenza virus 3

Swinepox virus

Vesicular stomatitis New Jersey virus

Porcine adenovirus

Herpes simplex virus 1

African swine fever virus

Pseudorabies virus

Vaccinia virus

CRL-2843   3D4/21  豬肺泡巨噬細(xì)胞

Swine vesicular disease virus

Comments

The plasmid carries the genes for neomycin resistance and SV40 large T antigen.


A subpopulation of each cell line (3D4/2 (ATCC CRL-2845), 3D4/21 (ATCC CRL-2843) and 3D4/31 (ATCC CRL-2844)) was positive, to varying degrees depending on the media formulation, for nonspecific esterase activity and phagocytosis.


Clone 3D4/21 can produce Bovine adenovirus type 3 (BAV-3) to markedly higher titers than clones 3D4/2 and 3D4/31.


Addition of DMSO improved the capability of clone 3D4/21 to replicate the field isolate of African swine fever virus (ASFV/Lillie) compared to the other clones.

Complete Growth Medium  RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, 1.0 mM sodium pyruvate supplemented with 0.1 mM nonessential amino acids, 90%; fetal bovine serum, 10%

Subculturing

Volumes used in this protocol are for 75cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w

) Trypsin - 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels. An inoculum of 5 x 103 to 7 x 103 viable cells/cm2 is recommended.

Incubate cultures at 37°C. Subculture when cell concentration reaches between 3 x 105 and 4 x 105 cells/cm2.

Subc*tion Ratio: A subc*tion ratio of 1:6 to 1:8 is recommended

Medium Renewal: Two to three times weekly

Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture Of Animal Cells: A Manual Of Basic Technique by R. Ian Freshney, 5th edition, published by Wiley-Liss, N.Y., 2005.


CRL-2843   3D4/21  豬肺泡巨噬細(xì)胞

Cryopreservation

Complete growth medium supplemented with 5% (v

) DMSO. Cell culture tested DMSO is available as ATCC® Catalog No. 4-X.

Culture Conditions

Temperature: 37°C

Atmosphere: 5% CO2 in air recommended























產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
2020中文字幕在线视频无码专区| 91人妻一区二区| 亚洲图片欧美激情一区| 欧美激情中出| 精品久久久久久无码囯产| 国产看片777777| 成人动作片免费一区| 免费视频涩涩| 免费无码AV不卡在线看| 国产高清美女在线| 91精品国产免费青青碰在线观看| 国产精品49页| 国产黄片在线看| 欧美亚洲日韩在线一区| 95国产精品自拍| 台湾人妻AⅤ三级片| 四虎影库在线免费| 久久久婷婷五月亚洲国产精品 | 久久香蕉国产线看观看精品蕉| 五月综合啪| 久久人妻无码精品| 综合网久久综合| 最新久色网| 亚洲av女人的天堂在线观看| 国产精品99一区不卡| 国产又吐又大成人版| 国产成人青青热久免费精品| 美日韩在线视频一区二区三区| 蜜臀美女日逼网站| 久久久久久久久无码精品亚洲日韩| 2020国产精品久香蕉在| 午夜男人在线播放| 香蕉久综| 日韩插啊免费视频在线观看| 又色又爽又黄的视频女女| 人妻初尝老外阅读| 久久久久久亚洲精品不卡性色av| 久久这里只有精品一区| 电影久久一区| 久久精品国产免费i| 国产人成精品香港三级在线|